Used for detecting and quantifying plant pectin methylesterase (PME) enzyme activity in agricultural and botanical research. Helps study cell wall modification during fruit ripening, plant development, and post-harvest storage. Widely applied in food science to assess texture changes in fruits and vegetables. Also supports research on plant-pathogen interactions and stress responses where PME activity plays a regulatory role. Provides a reliable, high-throughput method for enzyme analysis without requiring radioactive materials.