Used for the quantitative measurement of Plant Kunitz-type trypsin inhibitor (KO) activity in plant tissue extracts, seeds, and agricultural products. Helps assess anti-nutritional factors in feed and food crops, supporting research in plant breeding, nutritional quality improvement, and safety evaluation. Widely applied in agricultural labs, food safety testing, and biotechnology research to monitor protease inhibitor levels that may affect protein digestibility. Enables high-throughput screening of plant varieties for reduced anti-nutritional components.