Used to measure 15-lipoxygenase (15-LO/LOX) enzyme activity in mouse biological samples such as tissue homogenates, cell lysates, and serum. This assay helps in studying inflammatory processes, oxidative stress, and lipid metabolism, as 15-LOX is involved in the production of bioactive lipid mediators like 15-HETE and 13-HODE. Commonly applied in research on atherosclerosis, asthma, neurodegenerative diseases, and cancer to evaluate enzyme function and the impact of therapeutic interventions. Provides a quantitative, sensitive, and specific colorimetric readout for high-throughput analysis in preclinical studies.