Used to measure the activity of 6-phosphofructokinase (6-PFK) in human biological samples such as serum, plasma, or tissue extracts. This assay is commonly applied in research settings to study glycolytic metabolism, particularly in conditions where energy metabolism is altered, such as diabetes, cancer, and metabolic syndromes. It enables researchers to quantify enzyme activity levels accurately, supporting investigations into metabolic regulation, drug effects on glycolysis, and disease mechanisms involving impaired glucose metabolism. The kit is designed for high sensitivity and specificity, making it suitable for clinical research and laboratory diagnostics.