Used for the quantitative measurement of D-lactate dehydrogenase (D-LDH) enzyme activity in human biological samples such as serum, plasma, or tissue homogenates. This ELISA kit is particularly useful in clinical and research settings to assess mitochondrial function and cellular metabolism, as D-LDH plays a role in the conversion of D-lactate to pyruvate. Elevated D-LDH activity has been associated with metabolic disorders, bacterial infections, and conditions involving impaired oxygen utilization such as sepsis and lactic acidosis. The assay enables early detection and monitoring of disease progression by providing a sensitive and specific readout of enzyme activity, supporting diagnostic accuracy and evaluation of therapeutic interventions.