Used to measure α-glucosidase enzyme activity in biological samples such as serum, plasma, cell lysates, and tissue homogenates. Widely applied in research on metabolic disorders, particularly diabetes, where α-glucosidase plays a key role in carbohydrate digestion. Enables evaluation of enzyme inhibition for drug development and screening of potential antidiabetic compounds. Also used in studies of lysosomal storage diseases like Pompe disease, where α-glucosidase deficiency is a diagnostic marker. Provides a sensitive and specific quantitative analysis, supporting both clinical research and pharmaceutical testing.