Used in biomedical research to measure pyruvate kinase (PK) enzyme activity in human samples such as blood, serum, or cell lysates. It helps in studying metabolic disorders, especially those related to glycolysis, including hemolytic anemia caused by PK deficiency. The kit is widely used in clinical laboratories and research settings for accurate quantification of PK levels, aiding in diagnosis and monitoring of disease progression. It is also employed in drug development and metabolic studies to assess the impact of compounds on cellular energy metabolism.