Used to measure pyruvate carboxylase (PC) enzyme activity in human biological samples such as serum, plasma, or tissue extracts. This assay is commonly applied in metabolic research, especially in studies related to glucose metabolism, insulin secretion, and mitochondrial function. It helps in diagnosing and investigating metabolic disorders, including certain forms of lactic acidosis and inherited PC deficiencies. The kit is also utilized in clinical and research settings to evaluate metabolic changes in conditions like diabetes, obesity, and non-alcoholic fatty liver disease (NAFLD). Its high sensitivity and specificity make it suitable for monitoring enzyme activity in response to therapeutic interventions or genetic modifications.