Used in biomedical research to measure calcineurin (CaN) enzyme activity in human biological samples such as blood, serum, or tissue extracts. It helps in studying immune response regulation, especially in conditions involving T-cell activation. Commonly applied in research on autoimmune diseases, transplant rejection, and neurodegenerative disorders where calcineurin signaling plays a key role. The kit enables high-throughput analysis in clinical labs and pharmaceutical development for evaluating drug effects on calcineurin pathways.