Used to measure PI3K enzyme activity in biological samples such as cell lysates or tissue extracts. Commonly applied in research on cell signaling pathways, especially in studies related to cancer, insulin signaling, and immune responses. Enables researchers to assess the activation state of the PI3K pathway under different experimental conditions, including drug treatment or gene modification. Useful in drug development for screening compounds that modulate PI3K activity. Provides a quantitative and high-throughput method to evaluate pathway inhibition or activation without requiring radioactive materials.