Used in biomedical research to measure urokinase-type plasminogen activator (uPA) activity in human samples such as plasma, serum, or cell culture supernatants. Helps assess fibrinolysis, tumor progression, and metastasis in cancer studies. Commonly applied in clinical labs and pharmaceutical research to evaluate uPA system involvement in diseases like breast cancer, lung cancer, and cardiovascular conditions. Enables high-throughput screening and supports diagnostic development due to its sensitivity and specificity for active uPA forms.