Used to measure SIRT1 enzyme activity in human biological samples such as serum, plasma, or cell lysates. Commonly applied in research on aging, metabolic disorders, and neurodegenerative diseases. Helps evaluate the effects of drugs or compounds targeting sirtuin pathways. Enables high-throughput screening in drug development and biomarker studies. Provides quantitative results for monitoring changes in SIRT1 activity under different experimental or clinical conditions.