Used to measure mitogen-activated protein kinase (MAPK) activity in human cell or tissue samples. Commonly applied in research settings to study cell signaling pathways involved in inflammation, stress responses, proliferation, and apoptosis. Helps evaluate the effects of drugs, toxins, or genetic modifications on MAPK signaling. Widely used in pharmaceutical development, cancer research, and immunology to understand disease mechanisms and identify potential therapeutic targets. Provides quantitative data through colorimetric detection, enabling high-throughput screening in microplate format.