Used in biomedical research to measure tissue plasminogen activator (t-PA) activity in human blood or plasma samples. Helps assess fibrinolytic system function, which is critical in studying blood clotting disorders, thrombosis, and cardiovascular diseases. Commonly applied in clinical labs and research settings to evaluate the risk of stroke, deep vein thrombosis, or pulmonary embolism. Also used in monitoring patients undergoing thrombolytic therapy and in studies related to endothelial cell function.