Used to measure pyruvate kinase (PK) enzyme activity in human biological samples such as blood, plasma, or tissue extracts. This ELISA kit is commonly applied in clinical research and diagnostics to study metabolic disorders, especially those related to glycolysis. It is particularly useful in investigating conditions like hereditary hemolytic anemia, where PK deficiency leads to red blood cell destruction. The kit enables high-throughput, quantitative analysis of PK levels, supporting studies in cancer metabolism, as altered PK activity is often linked to tumor growth and energy metabolism in cancer cells. It is also used in drug development to assess the impact of therapeutic agents on cellular energy pathways.