Widely used in molecular biology and histochemistry as a chromogenic substrate for alkaline phosphatase detection. When cleaved by alkaline phosphatase, it produces a blue-insoluble precipitate at the site of enzyme activity, enabling visualization in techniques like immunohistochemistry, in situ hybridization, and enzyme-linked assays. Its distinct color development provides high contrast on biological tissues, making it ideal for precise localization of gene expression or protein targets. Commonly used in combination with other substrates for multiplex staining. Stable under standard assay conditions and compatible with various fixation methods.