The Glutaminase (GLS) Activity Assay Kit is widely used in research to measure the enzymatic activity of glutaminase, which plays a critical role in cellular metabolism. It is particularly valuable in cancer research, as glutaminase is often upregulated in tumor cells to support their rapid growth and energy demands. By quantifying GLS activity, researchers can study metabolic reprogramming in cancer and evaluate the efficacy of glutaminase inhibitors as potential therapeutic agents. Additionally, the kit is utilized in neuroscience to investigate the role of glutaminase in glutamate production, which is essential for neurotransmission and neuronal function. It is also applied in metabolic studies to understand glutamine metabolism in various tissues and its implications in diseases such as diabetes and neurodegenerative disorders. The assay provides a reliable and sensitive method for analyzing GLS activity in cell lysates, tissue homogenates, or purified enzyme preparations, making it a versatile tool for biomedical research.