The Glutaminase (GLS) Activity Assay Kit is widely used in biomedical research to measure the enzymatic activity of glutaminase, which plays a critical role in cellular metabolism. It is particularly valuable in cancer research, as glutaminase is often upregulated in tumor cells to support their rapid growth and energy demands. By quantifying GLS activity, researchers can study metabolic reprogramming in cancer and evaluate the efficacy of glutaminase inhibitors as potential therapeutic agents. Additionally, the kit is employed in neuroscience to investigate the role of glutaminase in glutamate production, which is essential for neurotransmitter regulation and synaptic function. It is also utilized in metabolic disorder studies to understand the enzyme's involvement in amino acid metabolism and its impact on diseases such as diabetes and obesity. The assay provides a reliable, high-throughput method for analyzing GLS activity in cell lysates, tissue homogenates, and purified enzyme preparations, making it a versatile tool for both basic and applied research.