The Mitochondrial Isocitrate Dehydrogenase (ICDHm) Activity Assay Kit is primarily used to measure the activity of ICDHm in biological samples. This enzyme plays a critical role in the tricarboxylic acid (TCA) cycle, where it catalyzes the conversion of isocitrate to alpha-ketoglutarate, generating NADH in the process. By assessing ICDHm activity, researchers can gain insights into mitochondrial function, cellular energy metabolism, and oxidative stress. This kit is particularly valuable in studies related to metabolic disorders, cancer research, and neurodegenerative diseases, where mitochondrial dysfunction is often implicated. Additionally, it is used in drug discovery and development to evaluate the effects of potential therapeutic compounds on mitochondrial activity. The assay is straightforward, providing accurate and reproducible results, making it a useful tool for both basic research and clinical applications.