The Aminopyrine-N-Demethylase (AND) Activity Assay Kit is used to measure the enzymatic activity of cytochrome P450 enzymes that catalyze the N-demethylation of aminopyrine in biological samples. This assay is essential in drug metabolism studies, enabling researchers to evaluate how xenobiotics, including drugs, are processed in the liver. It is commonly employed in pharmaceutical research to investigate drug interactions, metabolism pathways, and the influence of genetic or environmental factors on enzyme function. In toxicology, it helps assess the impact of chemicals or toxins on hepatic enzyme activity. The kit utilizes a spectrophotometric method to provide accurate quantification of enzyme activity, serving as a key tool in biochemical, pharmacological, and toxicological research.