The Isocitrate Lyase (ICL) Activity Assay Kit is primarily used in research to measure the activity of the enzyme isocitrate lyase, which plays a critical role in the glyoxylate cycle. This cycle is essential for the survival of certain microorganisms, including bacteria and fungi, under conditions where traditional carbon sources are limited. By assessing ICL activity, researchers can study microbial metabolism, particularly in pathogens like Mycobacterium tuberculosis, which relies on this pathway during infection. The kit is also valuable in screening potential antimicrobial drugs that target the glyoxylate cycle, offering insights into novel therapeutic strategies. Additionally, it aids in understanding plant metabolism, as the glyoxylate cycle is active in germinating seeds, facilitating the conversion of stored fats into carbohydrates. This makes the kit a versatile tool in both biomedical and agricultural research.