The Pyruvate Dehydrogenase (PDH) Activity Assay Kit is widely used in research and diagnostics to measure the activity of the pyruvate dehydrogenase complex, a critical enzyme in cellular metabolism. This kit is essential for studying mitochondrial function and energy production, as PDH plays a key role in converting pyruvate into acetyl-CoA, linking glycolysis to the citric acid cycle. It is commonly applied in studies related to metabolic disorders, diabetes, and cancer, where PDH activity is often dysregulated. Additionally, the kit is used in drug discovery to evaluate the efficacy of compounds targeting metabolic pathways. Its applications extend to neuroscience research, particularly in understanding neurodegenerative diseases associated with mitochondrial dysfunction. The kit provides a reliable and sensitive method for assessing PDH activity in various biological samples, including tissues, cells, and isolated mitochondria.