The α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit is primarily used to measure the activity of the α-KGDH enzyme in biological samples. This enzyme plays a critical role in the tricarboxylic acid (TCA) cycle, which is essential for cellular energy production. By assessing α-KGDH activity, researchers can evaluate mitochondrial function and metabolic health in various tissues. This kit is particularly useful in studying diseases associated with mitochondrial dysfunction, such as neurodegenerative disorders, metabolic syndromes, and cancer. It is also employed in drug discovery and development to screen compounds that modulate mitochondrial activity. The assay is straightforward, providing reliable and quantitative results, making it a valuable tool for both basic research and clinical applications.