The NAD-Malic Enzyme (NAD-ME) Activity Assay Kit is primarily used in research and diagnostic laboratories to measure the activity of the NAD-ME enzyme in biological samples. This enzyme plays a crucial role in the malate-aspartate shuttle, which is essential for the transport of reducing equivalents across mitochondrial membranes. By quantifying NAD-ME activity, researchers can gain insights into cellular metabolism, particularly in processes related to energy production and redox balance. This kit is valuable in studies focused on mitochondrial function, metabolic disorders, and plant physiology, where NAD-ME is involved in C4 photosynthesis. It is also employed in cancer research, as altered NAD-ME activity has been linked to tumor metabolism and progression. The assay is straightforward, providing reliable and reproducible results, making it a useful tool for both basic and applied research.