The NAD-Malic Enzyme (NAD-ME) Activity Assay Kit is primarily used to measure the activity of the NAD-ME enzyme in various biological samples. This enzyme plays a critical role in metabolic pathways, particularly in the malate-aspartate shuttle and the tricarboxylic acid (TCA) cycle, which are essential for energy production in cells. The kit is widely applied in research settings to study cellular metabolism, especially in plants, where NAD-ME is involved in C4 photosynthesis and respiration. It is also utilized in biomedical research to investigate metabolic disorders, mitochondrial function, and the impact of genetic modifications on enzyme activity. The assay provides a reliable and sensitive method for quantifying NAD-ME activity, aiding in the understanding of metabolic regulation and its implications in health and disease.