MTS Reagent is widely used in cell proliferation assays to measure the number of viable cells in culture. It is particularly valuable in research involving drug discovery, cancer studies, and toxicity testing. When added to cell cultures, the reagent is reduced by metabolically active cells, producing a colored formazan product. This color change is directly proportional to the number of living cells, allowing researchers to quantify cell viability and proliferation rates. The assay is non-radioactive, simple to perform, and provides rapid results, making it a preferred choice for high-throughput screening. Additionally, it is compatible with various cell types, including adherent and suspension cells, enhancing its versatility in biological and pharmaceutical research.