Used primarily for the cultivation and propagation of Brucella species, this broth supports the growth of fastidious, capnophilic organisms due to its rich nutrient composition. It is commonly employed in diagnostic laboratories for the isolation and identification of Brucella from clinical specimens such as blood, urine, synovial fluid, and tissue samples. The broth may also be used for antibiotic susceptibility testing and in research settings for maintaining viable cultures. When supplemented with serum or other growth enhancers, and incubated in an atmosphere with 5-10% CO2 or under microaerophilic conditions, it can improve recovery rates of slow-growing strains. Its application extends to veterinary diagnostics, where it aids in detecting infections in livestock, contributing to control and eradication programs for brucellosis, a zoonotic disease.