Used primarily in microbiology for the enrichment and cultivation of iron-oxidizing bacteria, especially those belonging to the genus Leptothrix and Gallionella. It supports the growth of bacteria that derive energy from the oxidation of ferrous iron (Fe²⁺) to ferric iron (Fe³⁺), forming visible iron oxide deposits. This broth is commonly used in environmental testing to detect iron bacteria in water systems, wells, and industrial pipelines where biofouling and corrosion are concerns. It helps in studying microbial-induced corrosion (MIC) and in assessing water quality. The medium is also useful in bioremediation research, where iron bacteria play a role in removing iron and manganese from contaminated water sources.