Carbamyl phosphate disodium salt is widely used in biochemical research, particularly in the study of enzyme kinetics and metabolic pathways. It serves as a substrate for enzymes like carbamoyl phosphate synthetase, which plays a critical role in the urea cycle and pyrimidine biosynthesis. This compound is essential for investigating the mechanisms of nitrogen metabolism and nucleotide synthesis in cellular systems. Additionally, it is utilized in laboratory experiments to produce carbamoyl aspartate, a key intermediate in the de novo synthesis of pyrimidines. Its stability and solubility make it a valuable reagent for in vitro studies and diagnostic applications related to metabolic disorders.