This compound is widely used in biochemical research, particularly in studies involving ATP-dependent processes. It serves as a photoreactive ATP analog, enabling the investigation of ATP-binding proteins and enzymes. The benzoylbenzoyl group allows for UV-induced cross-linking, making it a valuable tool for identifying and characterizing ATP-binding sites in proteins. It is also employed in mechanistic studies of ATPases, kinases, and other ATP-utilizing enzymes, providing insights into their function and regulation. Additionally, it is utilized in photoaffinity labeling experiments to study protein-ligand interactions and map active sites. Its applications extend to understanding cellular energy metabolism and signal transduction pathways.