Used extensively in biochemical research to study cellular energy transfer, enzymatic reactions, and metabolic pathways. It serves as a critical substrate in ATPase and kinase assays to understand enzyme mechanisms and kinetics. Applied in cell culture studies to maintain energy-dependent processes and support cell viability. Utilized in diagnostic kits for measuring ATP levels as an indicator of cell health or microbial contamination. Plays a role in synthesizing nucleic acids and proteins in vitro. Also employed in muscle physiology studies to investigate contraction and relaxation mechanisms.