This compound is widely used in biochemical research and enzymatic studies due to its role as a key intermediate in cellular energy metabolism. It serves as a substrate for various enzymes, including kinases and ATPases, making it essential for investigating energy transfer processes in cells. Additionally, it is utilized in the preparation of buffers and reagents for studying metabolic pathways, particularly those involving ATP synthesis and degradation. Its application extends to the development of diagnostic assays and therapeutic research, where understanding cellular energy dynamics is crucial.