3,5-Diazidobenzoic acid is primarily used in the field of organic synthesis and materials science. It serves as a key intermediate in the preparation of various azido compounds, which are valuable in click chemistry reactions, particularly the copper-catalyzed azide-alkyne cycloaddition (CuAAC). This reaction is widely employed for bioconjugation, polymer modification, and the development of functional materials. Additionally, the compound is utilized in the synthesis of energetic materials due to its azide groups, which can contribute to high-energy density applications. Its role in photochemistry is also notable, as it can be used in photoaffinity labeling to study protein-ligand interactions.