Dynole 2−24 is primarily used in research settings as a potent inhibitor of dynamin, a protein involved in endocytosis. Its application is significant in studying cellular processes such as vesicle formation and membrane trafficking. Researchers utilize this compound to investigate the role of dynamin in various physiological and pathological conditions, including synaptic transmission and cancer cell proliferation. Additionally, Dynole 2−24 serves as a valuable tool in drug discovery efforts aimed at targeting dynamin-related pathways for therapeutic purposes.