Pitstop 2 is widely used in biological research to study clathrin-mediated endocytosis, a critical cellular process. It specifically inhibits clathrin terminal domain interactions, making it a valuable tool for dissecting the role of clathrin in membrane trafficking and internalization of receptors. Researchers utilize Pitstop 2 to investigate the dynamics of endocytosis in various cell types, helping to understand its implications in diseases such as cancer, viral infections, and neurodegenerative disorders. Additionally, it serves as a control in experiments to validate the involvement of clathrin-dependent pathways in cellular uptake mechanisms. Its application extends to drug delivery studies, where it helps optimize nanoparticle and therapeutic agent uptake by cells.