Dyngo-4a is primarily used as a potent inhibitor of dynamin, a protein involved in cellular membrane fission processes, especially endocytosis. Its main application lies in research settings where it is used to block clathrin-mediated endocytosis, allowing scientists to study the role of endocytic pathways in various cellular functions. It is widely applied in cell biology and neuroscience to investigate internalization of receptors, virus entry into cells, and synaptic vesicle recycling. Due to its fast-acting and reversible effects, Dyngo-4a is favored in live-cell imaging and functional assays. It also serves as a tool compound in studies exploring the therapeutic potential of modulating endocytosis in diseases such as cancer, neurodegenerative disorders, and infectious diseases.