Pitstop 2 is primarily used in biological research to selectively inhibit clathrin-mediated endocytosis (CME), a critical cellular process for internalizing molecules and receptors. It is particularly valuable in studying the role of CME in various cellular functions, such as signal transduction, nutrient uptake, and membrane receptor regulation. Researchers use it to dissect the mechanisms of endocytosis and its impact on diseases like cancer, viral infections, and neurodegenerative disorders. Additionally, Pitstop 2 helps in distinguishing clathrin-dependent pathways from other endocytic routes, providing insights into targeted drug delivery systems and therapeutic strategies. Its application extends to live-cell imaging and functional assays, where it aids in visualizing and manipulating endocytic processes in real-time.