Dyngo-4a is widely used in cellular biology research as a potent inhibitor of dynamin, a protein crucial for endocytosis. Its primary application lies in studying the mechanisms of endocytic pathways, particularly clathrin-mediated endocytosis, by effectively blocking dynamin's GTPase activity. This allows researchers to investigate how cellular uptake processes, such as receptor internalization and synaptic vesicle recycling, are regulated. Dyngo-4a is also employed in neurobiology to explore neurotransmitter release and synaptic function, providing insights into neurological disorders. Additionally, it is used in virology to study viral entry mechanisms, as many viruses rely on endocytosis to infect host cells. Its specificity and efficacy make it a valuable tool for dissecting complex cellular processes and developing therapeutic strategies targeting endocytosis-related pathways.