Tricine-SDS-PAGE gel buffer is widely used in protein biochemistry for separating low molecular weight proteins and peptides. It is particularly effective for resolving proteins in the range of 1-100 kDa, offering better resolution compared to traditional Laemmli systems. This buffer is commonly employed in the analysis of complex protein mixtures, such as membrane proteins, which often have smaller sizes and require precise separation. Its application extends to the study of protein-protein interactions, post-translational modifications, and the characterization of recombinant proteins. Additionally, it is utilized in proteomics research for high-resolution gel electrophoresis, enabling detailed analysis of protein samples. The buffer's compatibility with downstream techniques like Western blotting further enhances its utility in molecular biology and biochemistry laboratories.