1X TG-SDS Buffer (Tris-Glycine-SDS) in premixed powder form is the standard running buffer used in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) for protein separation by molecular weight. It provides the appropriate ionic strength, pH (approximately 8.3), and SDS concentration to ensure consistent and efficient migration of denatured proteins through the polyacrylamide gel during electrophoresis. The premixed powder format allows for easy reconstitution into 1X working solution, minimizing preparation errors and saving time in lab protocols. This buffer is essential for molecular biology research, proteomics, clinical diagnostics, and biochemical analysis of protein samples.