Coomassie Brilliant Blue R-250 is widely used in biochemistry and molecular biology for staining proteins in polyacrylamide gels. It is a key component in the Bradford assay, a method for quantifying protein concentration in solutions. The dye binds to proteins, producing a color change that can be measured spectrophotometrically, allowing for accurate protein quantification. Additionally, it is employed in gel electrophoresis to visualize protein bands, aiding in the analysis of protein size, purity, and composition. Its sensitivity and ease of use make it a staple in laboratory settings for protein-related research and diagnostics.