N,N′-Methylenebis(acrylamide) is widely used as a crosslinking agent in the production of polyacrylamide gels, which are essential in various scientific and industrial applications. In biochemistry, it plays a critical role in the creation of electrophoresis gels, enabling the separation of proteins and nucleic acids based on their size and charge. This is particularly important in molecular biology research, diagnostics, and genetic analysis. Additionally, it is utilized in the synthesis of hydrogels for drug delivery systems, where its crosslinking properties help control the release of active pharmaceutical ingredients. The chemical is also employed in water treatment processes, where it aids in the formation of flocculants that remove impurities and suspended particles from wastewater. Furthermore, it finds applications in the production of adhesives, coatings, and specialty polymers, enhancing their mechanical strength and stability. Its versatility and effectiveness make it a valuable component in both laboratory and industrial settings.