Used for separating larger nucleic acid molecules, typically RNA or DNA fragments above 100 base pairs, in molecular biology research. Ideal for applications requiring high resolution of long nucleic acid sequences, such as RNA integrity analysis, Northern blotting, and electrophoretic separation of PCR products. The Tris-acetate buffer system provides stable pH and efficient conductivity during electrophoresis, ensuring consistent migration and sharp bands. Commonly used in gene expression studies, diagnostic assays, and quality control of nucleic acid samples. Compatible with downstream staining, blotting, and imaging techniques.