3-Hydroxy-acetaminophen is primarily used in pharmacological and toxicological research to study the metabolism of acetaminophen. It serves as a key biomarker to understand the pathways and mechanisms involved in the breakdown of acetaminophen in the body. Researchers utilize this metabolite to investigate liver function and the potential for hepatotoxicity, especially in cases of acetaminophen overdose. Additionally, it aids in the development of therapeutic strategies to mitigate liver damage by analyzing its formation and elimination. Its role in metabolic studies helps in assessing drug interactions and the impact of genetic variations on acetaminophen metabolism.