2-Oxo-Ivabradine hydrochloride is a key metabolite of ivabradine, used in the treatment of cardiovascular conditions such as chronic heart failure and stable angina. It plays a role in inhibiting the If current (hyperpolarization-activated cyclic nucleotide-gated channel current) in the sinoatrial node, contributing to heart rate reduction without significantly affecting myocardial contractility or blood pressure.

In pharmaceutical research and development, 2-Oxo-Ivabradine hydrochloride serves as a reference standard for pharmacokinetic analyses and studies on drug metabolism, particularly for assessing metabolite levels in plasma to better understand the efficacy and safety of ivabradine.

Additionally, it is important in drug manufacturing processes for quality control and purity assessment of ivabradine-containing products. It is also utilized in developing laboratory analytical methods, such as HPLC or LC-MS/MS, to quantify drug and metabolite levels in biological samples.