N,N'-Desethylene levofloxacin is a primary metabolite of levofloxacin, a fluoroquinolone antibiotic. It forms through N-deethylation in the body and is studied in pharmaceutical research to understand drug metabolism, elimination pathways, and potential pharmacological or toxicological effects of metabolites. This compound is also utilized in the development of analytical methods for detecting levofloxacin levels in biological samples such as blood or urine, aiding in therapeutic drug monitoring to optimize efficacy and minimize toxicity risks. Additionally, its structure supports investigations into quinolone derivatives for improved antimicrobial properties.