Used primarily in pharmaceutical research as a metabolite of apalutamide, N-Desmethyl Apalutamide helps in studying the metabolic pathways and pharmacological activity of apalutamide, a drug used in the treatment of prostate cancer. It serves as a reference standard in analytical testing to assess drug metabolism, bioavailability, and clearance in biological systems. Its presence in plasma indicates the extent of apalutamide metabolism in patients, aiding in dose optimization and therapeutic monitoring. It is also employed in mass spectrometry and pharmacokinetic studies to understand drug interactions and patient variability in response to treatment.