Used primarily in research settings to study the metabolic pathways and pharmacological effects of flupirtine, a former analgesic drug. Helps in understanding liver metabolism and neuroprotective mechanisms due to its active role as a major metabolite. Also utilized in toxicology studies to assess potential hepatotoxic effects associated with flupirtine use. Serves as a reference standard in analytical methods like HPLC and mass spectrometry for drug monitoring and forensic analysis.