5-hydroxy Propranolol is primarily utilized in research settings to study the metabolism and pharmacological effects of Propranolol, a widely used beta-blocker. It serves as a key metabolite in understanding how Propranolol is processed in the body, particularly in the liver, and its role in cardiovascular research. Additionally, it is employed in the development of analytical methods for detecting and quantifying Propranolol and its metabolites in biological samples, aiding in pharmacokinetic studies and drug monitoring. Its application also extends to investigating the relationship between drug metabolism and therapeutic efficacy or side effects.