The 5-fluoro AMB metabolite 3 is primarily used in research and analytical studies related to the metabolism of antifungal agents. It serves as a key marker in understanding the pharmacokinetics and metabolic pathways of antifungal compounds, particularly in the context of drug development and safety assessments. This metabolite is often analyzed in biological samples to evaluate the efficiency and breakdown products of antifungal treatments, aiding in the optimization of therapeutic dosages and minimizing potential side effects. Additionally, it plays a role in forensic and toxicological investigations to detect and quantify the presence of specific antifungal agents in biological systems.